Consulta: subjectFacets:"Leukemia"
Registros recuperados: 22
Data/hora: 19/05/2024 04:20:36

A never-ending story: the steadily growing family of the FA and FA-like genes
Provedor de dados: Genetics and Molecular Biology
Autores: Gueiderikh,Anna; Rosselli,Filippo; Neto,Januario B.C.
Abstract Among the chromosome fragility-associated human syndromes that present cancer predisposition, Fanconi anemia (FA) is unique due to its large genetic heterogeneity. To date, mutations in 21 genes have been associated with an FA or an FA-like clinical and cellular phenotype, whose hallmarks are bone marrow failure, predisposition to acute myeloid leukemia and a cellular and chromosomal hypersensitivity to DNA crosslinking agents exposure. The goal of this review is to trace the history of the identification of FA genes, a history that started in the eighties and is not yet over, as indicated by the cloning of a twenty-first FA gene in 2016.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Leukemia; Fanconi anemia; Chromosomal abnormalities.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300398

Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Lima,Melissa Pires de; Hilst,Luciana Farhat; Mattana,Fernanda Vanessa Rechinbach; Santos,Cid Aimbiré de Moraes; Weffort-Santos,Almeriane Maria.
The effects of the alkaloid-rich fraction of Himatanthus lancifolius (Müll. Arg) Woodson on normal marrow cells and leukemic cell lines were investigated. After 48 h exposure, the proliferation assay showed significant cell growth inhibition for Daudi (0.1-10 µg/mL), K-562 (1-10 µg/mL), and REH cells (10-100 µg/mL), yet was inert for normal marrow cells. A similar inhibition profile was observed in clonogenic assays. This alkaloid-rich fraction, in which uleine is the main compound, showed no signs of toxicity to any cells up to 10 µg/mL. Cell feature analyses after induction of differentiation showed maintenance of the initial phenotype. Flow cytometric expression of Annexin-V and 7-AAD in K-562 and Daudi cells has indicated that the cells were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apocynaceae; Cell proliferation; Himatanthus lancifolius; Indole alkaloids; Leukemia; Plant extract.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200014

CCAAT/enhancer-binding protein alpha (CEBPA) gene haploinsufficiency does not alter hematopoiesis or induce leukemia in Lck-CALM/AF10 transgenic mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lange,A.P.; Almeida,L.Y.; Araújo Silva,C.L.; Scheucher,P.S.; Chahud,F.; Krause,A.; Bohlander,S.K.; Rego,E.M.
Although rare, CALM/AF10 is a chromosomal rearrangement found in immature T-cell acute lymphoblastic leukemia (T-ALL), acute myeloid leukemia, and mixed phenotype acute leukemia of T/myeloid lineages with poor prognosis. Moreover, this translocation is detected in 50% of T-ALL patients with gamma/delta T cell receptor rearrangement, frequently associated with low expression of transcription factor CCAAT/enhancer-binding protein alpha (CEBPA). However, the relevance of CEBPA low expression for CALM/AF10 leukemogenesis has not yet been evaluated. We generated double mutant mice, which express the Lck-CALM/AF10 fusion gene and are haploinsufficient for the Cebpa gene. To characterize the hematopoiesis, we quantified hematopoietic stem cells, myeloid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; CEBPA; CALM/AF10; Transgenic mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600604

Collateral methotrexate resistance in cisplatin-selected murine leukemia cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bhushan,A.; Hacker,M.P.; Tritton,T.R.
Resistance to anticancer drugs is a major cause of failure of many therapeutic protocols. A variety of mechanisms have been proposed to explain this phenomenon. The exact mechanism depends upon the drug of interest as well as the tumor type treated. While studying a cell line selected for its resistance to cisplatin we noted that the cells expressed a &amp;gt;25,000-fold collateral resistance to methotrexate. Given the magnitude of this resistance we elected to investigate this intriguing collateral resistance. From a series of investigations we have identified an alteration in a membrane protein of the resistant cell as compared to the sensitive cells that could be the primary mechanism of resistance. Our studies reviewed here indicate decreased tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methotrexate transport; Resistance; Cisplatin; Leukemia; Phosphorylation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700005

Diagnostic-driven antifungal therapy in neutropenic patients using the D-index and serial serum galactomannan testing
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Garnica,Marcia; Sinhorelo,Aline; Madeira,Laura; Portugal,Rodrigo; Nucci,Marcio.
Abstract  Introduction Invasive mold disease is an important complication of patients with hematologic malignancies, and is associated with high mortality. A diagnostic-driven approach has been an alternative to the classical empiric antifungal therapy. In the present study we tested an algorithm that incorporated risk stratification using the D-index, serial serum galactomannan and computed tomographic-scan to guide the decision to start antifungal therapy in neutropenic patients.  Patients and methods Between May 2010 and August 2012, patients with acute leukemia in induction remission were prospectively monitored from day 1 of chemotherapy until discharge or death with the D-index and galactomannan. Patients were stratified in low, intermediate and high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal disease; Leukemia; Risk stratification; Neutropenia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400354

Differential effects of the methylenetetrahydrofolate reductase polymorphisms (C677T and A1298C) on hematological malignancies among Latinos: a meta-analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Garcia-Hernandez,Samanta Celeste; Meneses-Sanchez,Perla; Porchia,Leonardo Martin; Torres-Rasgado,Enrique; Pérez-Fuentes,Ricardo; Gonzalez-Mejia,Martha Elba.
Abstract Our objective was to determine the association between the methylenetetrahydrofolate reductase polymorphisms (C677T and A1298C) and the risk of developing acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML), acute myeloid leukemia (AML), and multiple myelomas (MM) in Latinos. PubMed, SCOPUS, EBSCO, LILACS, and other Latin-specific databases were searched for case-control studies that investigated the association between these polymorphisms and hematologic malignancies until November 2017. Genotype distributions were extracted and either fixed-effects or random-effects models were used to calculate the pooled crude odds ratios (ORs) for the heterozygous, homozygous, dominant, recessive, and allelic genetic models. No publication bias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MTHFR; Protective factor; Latin America; Leukemia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400549

Differential regulation of vitamin D receptor expression in distinct leukemic cell lines upon phorbol ester-induced growth arrest
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Folgueira,M.A.A.K.; Federico,M.H.H.; Roela,R.A.; Maistro,S.; Katayama,M.L.H.; Brentani,M.M.
A close correlation between vitamin D receptor (VDR) abundance and cell proliferation rate has been shown in NIH-3T3 fibroblasts, MCF-7 breast cancer and in HL-60 myeloblastic cells. We have now determined if this association occurs in other leukemic cell lines, U937 and K562, and if VDR content is related to c-myc expression, which is also linked to cell growth state. Upon phorbol myristate acetate (PMA) treatment, cells from the three lineages (HL-60, U937 and K562) differentiated and expressed specific surface antigens. All cell lines analyzed were growth inhibited by PMA and the doubling time was increased, mainly due to an increased fraction of cells in the G0/G1 phase, as determined by flow cytometry measurements of incorporated bromodeoxyuridine and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Calcitriol; Cell division; Cell differentiation; Leukemia; Phorbol esters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500011

Enhanced levels of the apoptotic BAX/BCL-2 ratio in children with acute lymphoblastic leukemia and high-risk features
Provedor de dados: Genetics and Molecular Biology
Autores: Kaparou,Maria; Choumerianou,Despoina; Perdikogianni,Chrysoula; Martimianaki,Georgia; Kalmanti,Maria; Stiakaki,Eftichia.
It has been suggested that leukemia is characterized by an impaired balance between the proliferation of blood cells and their capacity to undergo apoptosis. The aim of this study was to examine the expression of key molecules related to apoptosis (BCL-2, BAX, FAS, FAS-L) in children with acute lymphoblastic leukemia (ALL). Measurement of BCL-2 and BAX mRNA was performed by quantitative real-time PCR, and membrane expression of FAS and FAS-L was assessed by flow cytometry in bone marrow mononuclear cells, both at diagnosis and at remission following induction chemotherapy. At diagnosis, increased levels of the apoptotic BAX/BCL-2 ratio were observed in children older than 10 years and with higher white blood cell counts. A DNA index &lt; 1.16 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; Real-time PCR analysis; Protein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100002

Evaluation of ticarcillin/clavulanic acid versus ceftriaxone plus amikacin for fever and neutropenia in pediatric patients with leukemia and lymphoma
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Petrilli,Antonio Sérgio; Cypriano,Monica; Dantas,Lenice Silva; Lee,Lúcia Martino; Luisi,Maria Flávio Augusto Vercillo; Silva,Katia Veronica Torres B.; Pereira,Carlos Alberto Pires.
BACKGROUND: The empirical use of antibiotic treatments is widely accepted as a means to treat cancer patients in chemotherapy who have fever and neutropenia. Intravenous monotherapy, with broad spectrum antibiotics, of patients with a high risk of complications is a possible alternative. METHODS: We conducted a prospective open-label, randomized study of patients with lymphoma or leukemia who had fever and neutropenia during chemotherapy. Patients received either monotherapy with ticarcillin/clavulanic acid (T) or ceftriaxone plus amikacin (C+A). RESULTS: Seventy patients who presented 136 episodes were evaluated, 68 in each arm of the study. The mean neutrophil counts at admission were 217cells/mm³ (T) and 201cells/mm³ (C+A). The mean duration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fever; Neutropenia; Leukemia; Lymphoma; Ticarcillin; Ceftriaxone.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200003

Identification of clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers as a potential instrument for early detection of neoplasia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sales,M.M.; Bezerra,C.N.A.; Hiraki,Y.; Melo,N.B.; Rebouças,N.A.
We analyzed the genetic recombination pattern of the T-cell receptor beta-chain gene (TCR-beta) in order to identify clonal expansion of T-lymphocytes in 17 human T-lymphotropic virus type I (HTLV-I)-positive healthy carriers, 7 of them with abnormal features in the peripheral blood lymphocytes. Monoclonal or oligoclonal expansion of T-cells was detected in 5 of 7 HTLV-I-positive patients with abnormal lymphocytes and unconfirmed diagnosis by using PCR amplification of segments of TCR-beta gene, in a set of reactions that target 102 different variable (V) segments, covering all members of the 24 V families available in the gene bank, including the more recently identified segments of the Vbeta-5 and Vbeta-8 family and the two diversity beta segments....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human T-lymphotropic virus 1; Leukemia; Oligoclonal bands; T-cell receptor beta chain; Polymerase chain; Reaction; Adult T-cell leukemia/lymphoma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500006

MicroRNA-21 over expression in umbilical cord blood hematopoietic stem progenitor cells by leukemia microvesicles
Provedor de dados: Genetics and Molecular Biology
Autores: Razmkhah,Farnaz; Soleimani,Masoud; Ghasemi,Sorayya; Kafi-abad,Sedigheh Amini.
Abstract Microvesicles are able to induce the cell of origin’s phenotype in a target cell. MicroRNA-21, as an oncomir, is up-regulated in almost all cancer types such as leukemia which results in cell proliferation. In this study, we examine the ability of leukemia microvesicles to induce proliferation in hematopoietic stem progenitor cells (HSPCs) via microRNA-21 dysregulation. Herein, leukemia microvesicles were isolated from HL-60 and NB-4 cell lines by ultracentrifugation, and then their protein content was measured. Normal HSPCs were isolated from umbilical cord blood samples by a CD-34 antibody. These cells were treated with 20 and 40 μg/mL leukemia microvesicles for 5 and 10 days, respectively. Cell count, CD-34 analysis, and a microRNA-21 gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; Microvesicles; Hematopoietic stem cells; MicroRNA-21.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300465

miRNA expression profiles in chronic lymphocytic and acute lymphocytic leukemia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zanette,D.L.; Rivadavia,F.; Molfetta,G.A.; Barbuzano,F.G.; Proto-Siqueira,R.; Falcão,R.P.; Zago,M.A.; Silva-Jr,W.A.
MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles by targeting mRNAs for cleavage or translational repression. miRNAs act in diverse biological processes including development, cell growth, apoptosis, and hematopoiesis, suggesting their association with cancer. We determined the miRNA expression profile of chronic and acute lymphocytic leukemias (CLL and ALL) using the TaqMan® MicroRNA Assays Human Panel (Applied Biosystems). Pooled leukemia samples were compared to pooled CD19+ samples from healthy individuals (calibrator) by the 2-DDCt method. Total RNA input was normalized based on the Ct values obtained for hsa-miR-30b. The five most highly expressed miRNAs were miR-128b, miR-204, miR-218, miR-331, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Non-coding RNA; MicroRNA; Hematopoietic malignancies; Leukemia; MiR-331.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100003

Myeloproliferative syndrome of monosomy 7: a brief report
Provedor de dados: Genetics and Molecular Biology
Autores: Marques-Salles,Terezinha de Jesus; Soares-Ventura,Eliane Maria; Oliveira,Nathalia Lopes de; Silva,Mariluze; Assis,Reijane; Morais,Vera Lúcia Lins de; Otero,Luize; Fernandez,Teresa; Pombo-de-Oliveira,Maria do Socorro; Muniz,Maria Tereza Cartaxo; Santos,Neide.
We report the case of a five-month-old black male infant who had recurrent episodes of respiratory infections and also presented anemia and enlargements of the spleen, liver and lymphnodes. Hematological analysis revealed morphological abnormalities with megaloblastic dyserythropoiesis, while fetal hemoglobin assaying showed normal levels. Conventional and molecular cytogenetic analysis revealed monosomy of chromosome 7. Despite all therapeutic efforts during allogenic bone marrow transplantation, the child died due to generalized infection. The clinical and genetic distinctions between monosomy 7 syndrome and myelodysplastic disorders in childhood are discussed.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leukemia; Monosomy 7; Myeloproliferative syndrome.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100007

Ocorrência do vírus da leucemia felina em Felis cattus em Belo Horizonte
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Coelho,F.M.; Maia,M.Q.; Luppi,M.M.; Costa,E.A.; Luiz,A.P.M.F.; Ribeiro,N.A.; Bomfim,M.R.Q.; Fonseca,F.G.; Resende,M.
Blood samples from 1,072 domestic cats of nine administrative regions of Belo Horizonte, MG, were collected and tested using PCR nested for the occurrence of feline leukemia virus (FeLV). Overall occurrence was 47.5% (507/1072) being North (68.1%) and East (54.4%) the most prevalent areas. Epidemiological data showed that FeLV infection was very common among examined cats and breed neither gender nor were predisposing factors for FeLV. The results suggest that the agglomeration of a large number of cats in the same environment can be an important factor for the increase in the rate of transmission of this retrovirus among domestic cats in the studied city.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cat; Feline; Leukemia; Retrovirus; PCR-nested.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300037

Oral HPV infection in a bone marrow transplantation patient: a case report with atypical clinical presentation and unexpected outcome
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Pereira,Claudio Maranhão; Melo,Laura Guimarães; Correa,Maysa Milazzo; Souza,Cármino Antônio; Corrêa,Maria Elvira P.
HPV (Human Papilloma Virus) is one of the most prevalent infections worlwide. Oral HPV infection may be associated with different diseases of oral cavitie. Although oral HPV infection occurs frequently, it rarely causes lesions. An increased rate of oral HPV-induced lesions is observed in people with an impaired immune system. The most common conditions induced by oral HPV infection are focal epithelial hyperplasia, oral condylomas and oral papillomas. We reported a case of oral HPV lesion in a bone marrow transplantation patient with atypical clinical presentation and unexpected outcome.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: HPV; Bone marrow transplantation; Leukemia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100018

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608

Relationship of an hRAD54 gene polymorphism (2290 C/T) in an Ecuadorian population with chronic myelogenous leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Paz-y-Miño,César; López-Cortés,Andrés; Muñoz,María José; Castro,Bernardo; Cabrera,Alejandro; Sánchez,María Eugenia.
The hRAD54 gene is a key member of the RAD52 epistasis group involved in repair of double-strand breaks (DSB) by homologous recombination (HR). Thus, alterations of the normal function of these genes could generate genetic instability, shifting the normal process of the cell cycle, leading the cells to develop into cancer. In this work we analyzed exon 18 of the hRAD54 gene, which has been previously reported by our group to carry a silent polymorphism, 2290 C/T (Ala730Ala), associated to meningiomas. We performed a PCR-SSCP method to detect the polymorphism in 239 samples including leukemia and normal control population. The results revealed that the 2290 C/T polymorphism has frequencies of 0.1 for the leukemia and 0.1 for the control group. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Leukemia; CML; ALL; HRAD54; 2290 C/T polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400009

Silencing HIF-1α reduces the adhesion and secretion functions of acute leukemia hBMSCs
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Dong-Feng,Zeng; Ting,Liu; Cheng,Chang; Xi,Zhang; Xue,Liang; Xing-Hua,Chen; Pei-Yan,Kong.
Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenviroment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIF-1α; Human bone marrow stromal cells (hBMSCs); Leukemia; RNAi; SDF-1α; VEGF.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000004

The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chen,W.L.; Luo,D.F.; Gao,C.; Ding,Y.; Wang,S.Y.
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear cells (PBMCs) from 119 patients with de novo acute leukemia (AL) and 104 healthy controls, as well as in CD34+ cells from 12 AL patients and 10 healthy donors. A 429-bp fragment from a novel splicing variant of FAMLF was obtained, and a 363-bp consensus sequence was targeted to quantify total FAMLF expression. Kruskal-Wallis, Nemenyi, Spearman's correlation, and Mann-Whitney U-tests were used to analyze...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FAMLF; Gene expression; Leukemia; Real-time polymerase chain reaction; Alternative splicing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700603

The role of micro-ribonucleic acids in normal hematopoiesis and leukemic T-lymphogenesis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Slavov,S.N.; Gimenes Teixeira,H.L.; Rego,E.M.
Micro-ribonucleic acids (microRNAs) are small molecules containing 20-23 nucleotides. Despite their small size, it is likely that almost every cellular process is regulated by them. Moreover, aberrant microRNA expression has been involved in the development of various diseases, including cancer. Although many data are available about the role of microRNAs in various lymphoproliferative disorders, their impact on the development of acute lymphoblastic leukemia of T-cell progenitors is largely unknown. In this review, we present recent information about how specific microRNAs are expressed and regulated during malignant T-lymphopoiesis and about their role during normal hematopoiesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; Gene expression; Leukemia; Acute lymphoblastic leukemia; T cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700003

The ﬁrst reported catheter-related Brevibacterium casei bloodstream infection in a child with acute leukemia and review of the literature
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Bal,Zumrut Sahbudak; Sen,Semra; Karapinar,Deniz Yilmaz; Aydemir,Sohret; Vardar,Fadil.
Brevibacterium spp. are catalase-positive, non-spore-forming, non motile, aerobic Gram- positive rods that were considered apathogenic until a few reports of infections in immunocompromised patients had been published. To the best of our knowledge, this is the ﬁrst report of B. casei catheter-related bloodstream infection in a child with acute leukemia. We aim to enhance the awareness of pediatric hematology and infectious disease specialists about this pathogen and review of the literature.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Brevibacterium casei; Bloodstream infection Child; Leukemia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200213

TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Dalla Torre,C.A.; Maciel,R.M.B.; Pinheiro,N.A.; Andrade,J.A.D.; de Toledo,S.R.C.; Villa,L.L.; Cerutti,J.M.
Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Telomeric repeat amplification protocol; TRAP assay; Human thyroid cancer; Leukemia; Telomerase; Silver staining.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009